Cameroonian medicinal plants: a bioactivity versus ethnobotanical survey and chemotaxonomic classification

The study authors: Fidele Ntie-Kang, Lydia Likowo Lifongo, Luc Meva’a Mbaze, Nnange Ekwelle, Luc C Owono Owono, Eugene Megnassan, Philip N Judson, Wolfgang Sippl, Simon M N Efange.

(1) Chemical and Bioactivity Information Centre, Department of Chemistry, Faculty of Science, University of Buea (2) CEPAMOQ, Faculty of Science, University of Douala (3) Department of Chemistry, Faculty of Science, University of Douala (4) Klinikum Südstadt (5) Laboratory for Simulations and Biomolecular Physics, Ecole Normale Supérieure, University of Yaoundé I (6) Laboratory of Fundamental and Applied Physics, University of Abobo-Adjame (7) Chemical and Bioactivity Information Centre (8) Department of Pharmaceutical Sciences, Martin-Luther University of Halle-Wittemberg

Cameroonian medicinal plants: a bioactivity versus ethnobotanical survey and chemotaxonomic classification

In Cameroon herbs are traditionally used to meet health care needs and plans are on the way to integrate traditional medicine in the health care system, even though the plans have not been put into action yet. The country however has a rich biodiversity, with ~8,620 plant species, some of which are commonly used in the treatment of several microbial infections and a range of diseases (malaria, trypanosomiasis, leishmaniasis, diabetes and tuberculosis).


Our survey consisted in collecting published data from the literature sources, mainly from PhD theses in Cameroonian university libraries and also using the author queries in major natural product and medicinal chemistry journals. The collected data includes plant sources, uses of plant material in traditional medicine, plant families, region of collection of plant material, isolated metabolites and type (e.g. flavonoid, terpenoid, etc.), measured biological activities of isolated compounds, and any comments on significance of isolated metabolites on the chemotaxonomic classification of the plant species. This data was compiled on a excel sheet and analysed.


In this study, a literature survey led to the collection of data on 2,700 secondary metabolites, which have been previously isolated or derived from Cameroonian medicinal plants. This represents distinct phytochemicals derived from 312 plant species belonging to 67 plant families. The plant species are investigated in terms of chemical composition with respect to the various plant families. A correlation between the known biological activities of isolated compounds and the ethnobotanical uses of the plants is also attempted. Insight into future direction for natural product search within the Cameroonian forest and Savanna is provided.


It can be verified that a phytochemical search of active secondary metabolites, which is inspired by knowledge from the ethnobotanical uses of medicinal plants could be very vital in a drug discovery program from plant-derived bioactive compounds.


Biological activities, Chemotaxonomy, Ethnobotany, Medicinal plants, Natural products


The African continent holds an enormous resource in terms of floral biodiversity and its medicinal plants have remained a main reservoir of phytochemicals for pharmaceutical drug development1, 2. The above argument could be strengthened by the fact that the local populations, especially South of the Sahara, have depended on medicinal plants as the main source of remedy for the treatment of several medical disorders over the past centuries3. The World Health Organization (WHO) defines traditional medicine as practices, knowledge and belief systems which use minerals, plants and animal based remedies, spiritual therapies and exercises to prevent, treat and maintain well being4. According to WHO, 80% of the world’s population used natural remedies and traditional medicines4, 5. This is particularly the case in Africa, as well as in most developing countries, where a high proportion of the population still resorts to traditional medicine for their primary health care. Despite the advances in Western medicine (WM), African traditional medicine (ATM) has gained renewed interest in the health care services throughout the continent. This has probably been motivated by the rapidly increasing awareness of the potential and curative abilities of alternative medicines, especially from the use of medicinal plants, as well as the inadequate access to WM and physicians and the high cost for Western drugs6. ATM utilises medicinal plants which are traditionally taken as concoctions and infusions7. The argument for the local African populations resorting to traditional remedies could also be partly justified by the fact that natural product inspired molecules represented 80% of drugs that had been put into the drug market by 19908, 9, 10. The above figures have been the motivating factor behind the efforts of several research teams spread across the African continent, which have been actively engaged in an ethnobotanical/bioassay-guided search for active principles from medicinal plants which have been employed in ATM. The aim has been to extract, isolate and structurally characterise plant metabolites which could be developed into modern therapeutic agents. This process has often been coupled with biological screening of the isolated compounds, with a view to validating the uses of the plants in traditional medicine, thus identifying the molecular structures that could be further developed into drugs. Very often, success in this endeavour could be attributed to the closeness of the collaboration between experts in various fields like traditional healers, ethnobotanists, plant taxonomists, phytochemists, and biochemical screeners11. Since ATM often employs the preparation of macerations, preparation of decoctions of stem barks, leaves and roots, burning of leaves and using the derived ashes, etc., a rather straightforward procedure could be to test for the activity of the crude extracts of these plant parts. Such research has been reported and the results for activity as well as the toxicity of crude extracts serve as a guide towards the development of “total” extracts which are often directly conditioned and used in the low cost “modern” traditional treatments, affordable to the local populations12. Such approaches could however present serious drawbacks when situated within a modern context of WM. In WM, modern pharmaceutical companies have elaborated expensive Research and Development (R&D) procedures that work with highly sophisticated solid support combinatorial synthesis followed by highthroughput screening (HTS), in vivo animal model evaluations and clinical trials in order to determine appropriate doses, modes of administration and possible side effects before marketing the finished products (drugs). It could however be noted that one of the strongest arguments why ATM has become the main stay in the health care system of the continent, despite the above mentioned drawbacks, is that its local populations are often incapable of purchasing the expensive Western drugs13.In Cameroon, located in the core of the continent, and bounded by Nigeria (to the West), Chad (to the North and North East), Central Africa Republic to the South East and Gabon, Congo and Equatorial Guinea to the South (Figure 1), the picture is not very different from what is seen throughout the rest of the continent. The mortality patterns reflect high levels of infectious diseases and the risk of death during pregnancy and childbirth, in addition to cancers, cardiovascular diseases and chronic respiratory diseases which account for most deaths in the developed countries14. It has been reported that only 3 out of 20 patients are able to buy prescribed drugs in hospitals and only 1 out of every 1000 patients is able to consult a specialist15. As a result there is a rich tradition in the use of herbal medicines for the treatment of several ailments and plans are on the way to integrate traditional medicine in the health care system, even though the plans have not been put into action yet16. Cameroon however has a rich biodiversity, with ~8,620 plant species17, 18, some of which are commonly used in the treatment of several microbial infections19 and a range of neglected tropical diseases, including malaria, trypanosomiasis, leishmaniasis, diabetes, tuberculosis, etc.15. Adjanohoun et al. have provided a useful review on a collection of 414 plants used in traditional medicine in Cameroon, belonging to 95 plant families20. However, in addition to the fact that the formulations of the plant materials to be used as drugs in ATM have not been validated in a well documented and universally accepted pharmacopoeia, no details are given regarding the chemical composition of these plants. Jiofack et al. added to this by providing the formulation for 289 plant species belonging to 89 families, which have been used in two ethnoecological regions (Littoral-South West, and Sudano-Sahelian regions) of Cameroon for the treatment of various ailments, including amoebiasis, boils, cough, dermatitis, diarrhoea, dysentery, fever, gastritis, gonorrhoea, malaria, male sexual disorders, ovarian cysts, rheumatism, sexually transmitted diseases, sterility, syphilis, typhoid, and wounds21, 22, 23. Jiofack et al. also added a list of 140 plant species belonging to 60 families, which are used to treat a wide range of ailments in the upper Nyong valley forest in Cameroon24. Simbo also carried out an ethnobotanical survey around Babungo in the North West region of Cameroon, in which 107 plant species from 54 plant families (mostly from the Asteraceae family), used for the treatment of 55 health problems, were identified25, while Focho et al. identified 82 species of trees belonging to 70 genera and 42 families used by the local people in traditional medicine around Fundong in the North West region to treat 48 human ailments26. Ngono et al. carried out a survey in which plants used in the treatment of viral diseases in the Centre and South regions were identified7. Noumi et al. identified 26 plant species used to treat hypertension in the Bafia area of the Centre region27, 22 medicinal plant species used in the treatment of urinary lithiasis in the Littoral region28, 29 plants from 24 families used in the treatment of asthma in the Nkongsamba region29, 29 medicinal plants used in the treatment of peptic ulcers around Bagangte in the West region30, as well as 50 plants from 33 families which are used in the treatment of syphilis in Ebolowa in the South region, Cameroon31. Mpondo et al. conducted an ethnobotanical survey among the Douala communities and identified more than 100 genera which have been used in the treatment of diverse diseases like stomach ache, tooth ache, diabetes, cough, yellow fever, amoebic dysentery, anaemia, intestinal worms, fever and diarrhoea32, 33, 34, 35, 36, 37, 38. Ngo Bum et al. also reported 23 plants species used in Cameroon and Central Africa to treat epilepsy39, while Betti identified 102 plants from 97 genera and 51 families used mainly to treat cough, lactation failure, malaria, wounds, and toothache among the Baka Pygmies in the Dja biosphere reserve40. Sandberg et al. have also reported a collection of 32 botanically identified medicinal plants from the Mount Cameroon slope, made by Swedish settlers at the beginning of the last century, which has been donated to the Karolinska Institutet, Sweden41. In all the above cases, no details were given about the active principles contained in these plants, which render them useful in ATM. Titanji et al. have however previously presented a review of about 217 cited species used in the treatment of malaria in Cameroon, among which about 100 potential leads for the development of new antimalarials had been isolated from 26 species42.

Figure 1

Cameroon (in brown) and its surrounding neighbours.

Figure 1 Cameroon (in brown) and its surrounding neighbours.

In a recent paper, we have reported the results of a survey of the chemical composition of 234 plant species (representing 55 families) from the Cameroonian rainforest, from which 1,859 distinct compounds had been isolated or derived43. Our focus had been to highlight the medicinal value and virtues of the isolated phytochemicals by discussing the various measured biological activities and evaluating the “drug-like” properties by use of Lipinski’s “Rule of Five”44. In the current paper, we have enriched the data collection to include phytochemicals from 312 distinct plant species from 67 families, for which biological activities of 46.3% of the 2,769 pure compounds have been determined, and among which 32.7% of the compounds were identified or derived from Cameroonian medicinal plants for the very first time. In addition, we present and discuss 98 compounds whose measured biological activities correlated well with the ethnobotanical uses of the plants in ATM. An attempt to relate the chemical composition of the plant species to the families and genera is also carried out. From our study, it can be verified that a phytochemical search of active secondary metabolites, which is inspired by knowledge from the ethnobotanical uses of medicinal plants could be very vital in a drug discovery program from plant-derived bioactive compounds.


Data sources and collection methods

The plant sources, geographical collection sites, chemical structures of pure compounds as well as their spectroscopic data, were retrieved from literature sources comprising of data collected from 30 PhD theses, 556 articles from 75 peer reviewed journals, 7 unpublished conference presentations (from personal communication with the authors) and 2 textbook chapters, spanning the period 1971 to 2013. A full list of journals consulted is given in the supplementary material (Additional file1). Our survey consisted in collecting published data from the literature sources, mainly from PhD theses in Cameroonian university libraries and also using the author queries in major natural product and medicinal chemistry journals. The collected data includes plant sources, uses of plant material in traditional medicine, plant families, region of collection of plant material, isolated metabolites and type (e.g. flavonoid, terpenoid, etc.), measured biological activities of isolated compounds, and any comments on significance of isolated metabolites on the chemotaxonomic classification of the plant species (as commented in the literature). This data was compiled on a excel sheet and analysed.

Results and discussion

Chemotaxonomy of Cameroonian medicinal plants

Chemotaxonomy or chemosystematics is the attempt to classify and identify organisms (originally plants), according to demonstrable differences and similarities in their biochemical compositions. Generally speaking, it has been observed that plants of the same family usually synthesize compounds of similar classes due to the presence of similar classes of enzymes and hence similar biosynthetic pathways. Moreover, the medicinal plants surveyed are a complex of species from different groups (genera or families). This paper is not intended to give an accurate description of the chemotaxonomy of Cameroonian medicinal plants. The similarities or differences of chemical components from different medicinal plants presented herein are solely based on data published so far, by presenting trends towards the full description of the taxonomy of the studied families and species.
Our initial collection was composed of 3,742 phytochemicals previously isolated from 67 families, along with 319 of some of their hemisynthetic products, giving a total of 4061 chemical structures. Removal of duplicates gave 2,770 pure compounds. In our analyses, emphasis was laid on those plant families from which at least 2.5% of the secondary metabolites have been isolated. These include, by order of merit, Leguminosae (13.9%), Moraceae (10.6%), Guttiferae (10.1%), Rutaceae (6.5%), Meliaceae (4.5%), Euphorbiaceae (4.4%), Compositae (3.9%), Zingiberaceae (3.4%), Ochnaceae (3.2%), Bignioniaceae (3.1%), Sapotaceae (3.1%) and Apocynaceae (2.8%), Figure 2. An overall distribution by compound type (based solely on unique compounds, not compound concentrations in the plants) is shown in Figure 3. This revealed that terpenoids were most abundant in Cameroonian medicinal plants (constituting 26.0% of the isolated compounds). This was followed by flavonoids (19.6%), alkaloids (11.8%), xanthones (5.4%), quinones (5.0%) and glycosides (4.9%), showing a similar trend with our previous analysis of 1,859 metabolites43.

Figure 2

Bar chart showing the distribution of isolated compounds by plant family. Family names are indicated by the first four letters, e.g., Apoc = Apocynaceae.

Figure 2 Bar chart showing the distribution of isolated compounds by plant family. Family names are indicated by the first four letters, e.g., Apoc = Apocynaceae.

Figure 3

Pie chart showing the distribution by compound types.

Figure 3 Pie chart showing the distribution by compound types.

A more detailed analysis of compounds isolated per family is given in Table 1. From the Leguminosae family, it was shown that most of the isolated metabolites were flavonoids (68.3%). A similar trend was seen in the Ochnaceae and Moraceae families with respective flavonoid % compositions of 69.2% and 39.7%. The terpenoid rich families are the Meliaceae (74.4%), the Euphorbiaceae (68.5%), the Sapotaceae (43.2%) and the Zingiberaceae (70.1%), while the alkaloid rich families are the Apocynaceae (54.4%) and the Rutaceae (54.6%). From our analyses it was shown that, as in our previous paper, most of the isolated metabolites from the Guttiferae family were xanthones and quinines43. Moreover, within the Leguminosae family, isoflavonoids and diterpenoids, with insecticidal, piscicidal, and molluscicidal properties, are taxonomic markers of the genus Millettia45. Meanwhile within the Bignoniaceae family, sterequinone F (1), ursolic acid (2), triacontan-1,30-dioldiferulate (3) and p-coumaric acid (4) have been identified as the taxonomic markers for the genus Stereospermum, while the glycoside eutigoside A (5) has been used to differentiate S. accuminatissimum from other members of the genus46, 47, 48. The chemical structures of the taxonomic markers are shown in Figure 4. Within the Guttiferae family, laurentixanthone C (6) was identified to be the chemotaxonomic marker of the genus Vismia49. As for the Moraceae family, the coumarins psoralen, bergapten, 7-hydroxycoumarin and 7-methoxycoumarin (7 – 10 respectively) and lupeol (11) have been identified as taxonomic markers for the Dorstenia genus50. It was however generally observed that species of the genus Dorstenia harvested from across the African continent are particularly unique in that they produce a wide range of chalcones and bichalcones, prenylated flavonols, benzofuran derivatives, and furocoumarins51. It was also noticed that 6,9-dihydro-megastigmane-3-one (12) has been isolated from the genus Treculia (both T. africana and T. Acuminata). This compound may tentatively be considered as a marker of the Treculia genus. Isobavachalcone (13), previously isolated from several species of the genus Dorstenia, can be used to establish intertribal relationship between the two genera Treculia and Dorstenia52. Moreover, the isolation of compounds 8, 14 and 15 from Dorstenia and Ficus species indicate that these compounds could be chemotaxonomic markers for the Dorstenia genus and confirms that the genera Dorstenia and Ficus are closely related taxonomically53. Regarding the Ochnaceae, the biflavonoid amentoflavone (16) could be as well regarded as the taxonomic marker of the genus Ouratea, ochnaflavone (17) used to identify species of both Ouratea and Ochna, while lophirone A (18) could be associated with almost all genera in the family54, 55. Meanwhile, the presence of the flavonoid luteolin (19) in Lophira alata could be used to distinguish between the two species L. alata and L. Lanceolata56. Among species of the Rutaceae family, the accumulation of acridone alkaloids displays a common biogenetic trend in the genus Citrus, and could be used to distinguish this genus from the counterpart Afraegle57. Meanwhile, within this same family, the unique presence of the tetranortriterpenoids (20–23) within the genus Clausena, could reveal that these compounds are of taxonomic interest58. Within the Zingiberaceae family labdane diterpenes may represent a chemotaxonomic marker of the genus Aframomum. However, A. arundinaceum is one of the few species of Aframomum from which sesquiterpenoids are reported59, 60.

Table 1

Summary of the chemical composition of the remarkable plant families with abundant phytochemicals isolated

Plant family% of isolated compoundsRemarkable compound classes (% composition)Genera studied
Apocynaceae2.8Alkaloids (54.4%)Holarrhena, Picralima, Tabernathe, Voacanga, Picralima, Rauvolfia and Plumeria
Bignoniaceae3.1Quinones (26.3%)Kigelia, Newbouldia, Spathodea and Stereospermum
Compositae3.9Terpenoids (54.1%)Chromoleana, Crassocephalum, Crepis, Echinops, Elephantopus, Eupatroium, Helichrysum, Microglossa, Senecio, Tithonia and Vernonia
Euphorbiaceae4.4Terpenoids (68.5%)Alchornea, Antidesma, Croton, Discoglypremna, Drypetes, Fontainea, Macaranga, Maesobotrya, Neoboutonia, Thecacoris and Uapaca
Guttiferae10.1Xanthones (37.5%), Quinones (26.3%)Allanblackia, Calophyllum, Endodesmia, Garcinia, Harungana, Hypericum, Pentadesma, Psorospermum, Symphonia and Vismia
Leguminosae13.9Flavonoids (68.3%)Cassia, Crotalaria, Eriosema, Erythrina, Guibourtia, Millettia, Tephrosia and Piptadenia
Meliaceae4.5Terpenoids (74.4%)Carapa, Entandrophragma, Leplaea, Pterorhachis, Synsepalum, Trichilia and Turraeanthus
Moraceae10.6Flavonoids (39.7%)Antiaris, Artocarpus, Dorstenia, Ficus, Melicia, Milicia, Morus, Treculia and Trilepisium
Ochnaceae3.2Flavonoids (69.2%)Campylospermum, Lophira, Ochna and Ouratea
Rutaceae6.5Alkaloids (54.6%)Afraegle, Araliopsis, Basalmocitrus, Citropsis, Clausena, Fagara, Oricia, Oriciopsis, Teclea, Vepris, and Zanthoxylum
Sapotaceae3.1Terpenoids (43.2%)Butyrospermum, Chrysophyllum, Donella, Gambeya and Mimusops
Zingiberaceae3.4Terpenoids (70.1%)Aframomum and Renealmia

Figure 4

Chemical structures of taxonomic markers

Figure 4 Chemical structures of taxonomic markers.

Bioactivity versus ethnobotanical uses

Ethnobotany is the study of how modern and indigenous societies view and use plants61. As previously mentioned, medicinal plants from Cameroon have been used to treat a number of ailments. In this study, our focus is on those plants from which bioactive secondary metabolites have been isolated, which validate their ethnobotanical uses. Table 2 shows a summary of the results of biological activities of the isolated compounds, which correlate with the ethnobotanical uses of the plants (while the chemical structures of the bioactive metabolites are shown in Figures 5,6,7 and8). In each case, the significant uses and corresponding bioactivities are in bold characters. The activities of the selected metabolites are anti-malarial, estrogenic, antisalmonellal, hepatoprotective, antifungal, antioxidant, antidermatophytic, vasorelaxant, anticancer, antileishmanial, antimicrobial and α-glucosidase inhibition. The antimalarials with significant antiparasitic activity, which have been isolated from plants used in anti-malarial or anti-fever preparations include: betulinic acid (49), 2,2’,5,6’-tetrahydroxybenzophenone (50), 5-hydroxy-3-methoxyxanthone (51) and 3-hydroxy-5-methoxyxanthone (52)62; bazouanthrone (54), ferruginin A (55), harunganin (56), harunganol A (57) and harunganol B (58)63; isoxanthochymol (59), which exhibited an anti-malarial activity against the NF54 strain with a 50% inhibitory concentration (IC50) of 2.21 μM64; gaboxanthone (65), symphonin (66), globuliferin (67) and guttiferone A (68)65; garcinone E (70), with an IC50 of 0.20 μM, which was isolated from Pentadesma butyracea, concurrently with other potent anti-malarials pentadexanthone, cratoxylone and α-mangostin66; the limonoid 7α-obacunyl acetate (80)67; the homogentisic acid derivatives methyl 2-(1’β-geranyl-5’β-hydroxy-2’-oxocyclohex-3’-enyl) acetate (87) and 2-(1’β-geranyl-5’β-hydroxy-2’-oxocyclohex-3’-enyl)acetic acid (88) and the alkaloid liriodenine (89) isolated from Glossocalyx brevipes66; the flavonoids artocarpesin (90), Kushenol E (91), and the arylbenzofuran derivative mulberrofuran F (92) isolated from Morus mesozygia68; the chalcones bartericin A (97) and 4-hydroxylonchocarpin (100)69; 3-O-betulinic acid p-coumarate (112) isolated from Baillonella toxisperma, with an IC50 of 1.65 μM70; the labdane 3-deoxyaulacocarpin A (115) from Aframomum zambesiacum71; and the sesquiterpenoids oplodiol (118), 5E,10(14)-germacradien-1β,4β-diol (119) and 1(10)E,5E-germacradien-4α-ol (120) with respective IC50 values of 4.17, 1.63 and 1.54 μM72. In all cases, antiplasmodial activity was measured by inhibition of the chloroquine-resistant W2 P. falciparum strain with IC50 < 5 μM.

Figure 5

Chemical structures of bioactive metabolites I. Compounds 24 to 52.

Figure 5 Chemical structures of bioactive metabolites I. Compounds 24 to 52.

Figure 6

Chemical structures of bioactive metabolites II. Compounds 53 to 79, 82 and 93

Figure 6Chemical structures of bioactive metabolites II. Compounds 53 to 79, 82 and 93.

Chemical structures of bioactive metabolites II. Compounds 53 to 79, 82 and 93.

Figure 7

Chemical structures of bioactive metabolites III. Compounds 80, 81, and 83 to 106

Figure 7 Chemical structures of bioactive metabolites III. Compounds 80, 81, and 83 to 106.

Table 2

Summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites

Brillantaisia lamium, Crinum purpurascens, Senecio mannii, Stereospermum zenkeri, Ceiba pentandra, Cassia petersiana, Pteleopsis hylodendron, Terminalia superba, Diospyros canaliculata, Hypericum lanceolatum, Harungana madagascariensis, Garcinia Polyantha, Allanblackia gabonensis, Symphonia globulifera, Psorospermum febrifugum, Pentadesma butyracea, Irvingia gabonensis, Millettia griffoniana, Erythrina sigmoidea, Entandrophragma angolense, Turraeanthus africanus, Cylicodiscus gabunensis, Albizia adianthifolia, Glossocalyx brevipes, Morus mesozygia, Antiaris africana, Treculia obovoidea, Artocarpus communis, Dorstenia barteri, Ficus cordata, Pycnanthus angolensis, Coula edulis, Baillonella Toxisperma, Vitex cienkowskii, Aframomum zambesiacum, Reneilmia cincinnata.

Plant familyPlant speciesEthnobotanical usesPart of plant studiedIsolated metabolitesMeasured activity of Isolated metabolite
AcanthaceaeBrillantaisia lamiumThe aerial part of this plant is used in the treatment of various microbial infections, such as skin diseases and infections of the urinary tract.aerial parts11, 24, 25 and 26Antimicrobial, Tamokou et al.73
AmaryllidaceaeCrinum purpurascensThe macerated leaves are used as anti-poison or as antidote of mystical poisoning. Also, an infusion of the leaves is used for the treatment of some microbial infections.leaves27 and 28Anti-salmonellal and antibacterial, Nkanwen et al.74
AsteraceaeSenecio manniiThe plant is used to treat microbial and fungal infections.aerial parts29 and 30Antifungal, Ndom et al.75
BignoniaceaeStereospermum zenkeriThe bark is to treat bronchitis; its roots and leaves are used to cure fever and microbial infections.stem bark31 and 32Antimicrobial, Lenta et al.48
BombacaceaeCeiba pentandraThe plant is well reputed in for the treatment of many illnesses, such as, headache, dizziness, constipation, skin diseases, mental troubles, and fever.stem bark33 and 34Antifungal, Ngounou et al.76
CaesalpiniaceaeCassia petersianaThe leaves are generally used for the treatment of typhoid fever.leaves35, 36, 37, 38, 39 and 40Antisalmonellal activity, Djemgou et al.77, Gatsing et al.78
CombretaceaePteleopsis hylodendronTreatment of STDs, female sterility, kidney and liver disorders and dropsy.bark41, 42, 43 and 44Active against Staphylococcus aureus , S . pyogenes , and Baccilus cereus, Rahman et al.79
Terminalia superbaUsed to treat gastroenteritis, diabetes, female infertility and abdominal pain.bark45 and 46α-Glycosidase inhibition, immunoinhibitory activity, Tabopda et al.80
EbenaceaeDiospyros canaliculataUsed in the treatment of whooping cough, leprosy, snake bites, scabies, skin eruptions, dysentery, eye infections, menstrual troubles, abdominal pains, wounds, ulcers, chest pains and skin infections.stem bark11, 47 and 48Antifungal activity, Dzoyem et al.81
Hypericaceae, Clusiceae or GuttiferaeHypericum lanceolatumUsed to treat several ailments including malaria, skin infections, venereal diseases, gastrointestinal disorders, tumours, and infertility, epilepsies and nerves problems.stem bark49, 50, 51 and 52Anti-malarial, Zofou et al.62
Harungana madagascariensisEffective in the treatment of jaundice, diarrhoea, dysentery, typhoid fever, and constipation. Decoction of leaves is also used in liver problems and against anaemia. The roots and bark are used to treat malaria.stem bark49, 53, 54, 55, 56, 57 and 58Antiplasmodial, Lenta et al.63
Hypericaceae, Clusiceae or GuttiferaeGarcinia PolyanthaThe plant has some anti-malarial property. The yellow resinous sap (latex) is used to make a dressing for wounds.root bark59This compound shows anti-malarial activity by strong chemosuppression of parasitic growth, Lannang et al.64
Allanblackia gabonensisUsed against infections like dysentery, cold, and toothache.stem bark60, 61, 62, 63 and 64Activity against Leishmania amazonensis and antimicrobial activities against a range of Gram-negative and Gram-positive bacteria, Azebaze et al.82
Symphonia globuliferaUsed to cure several diseases such as stomach and skin aches. It is also used as laxative for pregnant women and as a general tonic. The bark is used by traditional healers to treat malaria.seed shells65, 66, 67 and 68Anti-malarial, antioxidant, Ngouela et al.65
Psorospermum febrifugumPlants of this genus are largely used in the African folk medicine as febrifugal, antidote against poison and purgative. They are also used as a remedy for the treatment of leprosy, skin diseases (such as dermatitis, scabies and eczemas) and subcutaneous wounds.roots69Antimicrobial (bacteria and fungi), Tsaffack et al.83
Pentadesma butyraceaDifferent parts of the plant are used in tropical African medicine to treat fever, coughs, constipation, bronchitis, and venereal diseases and viral infections.fruit pericarp70Erythrocyte susceptibility, antiplasmodial, Lenta et al.84
IxonanthaceaeIrvingia gabonensisThe stem bark decoction is used in the treatment of gonorrhoea, gastrointestinal or hepatic disorders, as a purgative, as well as a host of ailments. The decoction of the root barks is also used to treat diarrhoea and as mouth bath in the dental neuralgias.stem bark49, 53, 71, 72, 73 and 74Antimicrobial. Compound 74 is particularly active against Neisseria gonorrhoeae, confirming the ethnobotanical use of the plant in the treatment of the disease caused by this agent, Kuete et al.85
stem bark53
Hepatoprotective activity. This crude extract and isolated compound 53 might be useful for the prevention of toxic-induced and free radical-mediated liver diseases, since it has been suggested that compounds may be used as prophylactic agents, Donfack et al.86
Leguminosae-PapilionoideaeMillettia griffonianaCrude extracts from root and stem bark are used to treat boils, insect bites, inflammatory affections like pneumonia and asthma, sterility, amenorrhea and menopausal disorders.root bark75 and 76Estrogenic activity, Wanda et al.45
Erythrina sigmoideaWidely used in Cameroon to treat syphilis, wounds of ulcers and female sterility.stem bark77, 78 and 79Antibacterial activity against Staphylococcus aureus, Kouam et al.87, 88, 89
MeliaceaeEntandrophragma angolenseUsed as an anti-malarial or antipyretic in traditional medicine.stem bark80 and 81Antiplasmodial, Bickii et al.67
Turraeanthus africanusTreatment of typhoid fever.seeds82Antisalmonellal activity against Salmonella typhi, S. paratyphi A and S. paratyphi B, Djemgou et al.90
The species of this genus have been used for the treatment of cardiovascular disease, stomach ache, rheumatism pains, and asthma. The stem bark is used in the treatment of intestinal worms.seeds82Antimicrobial, Djemgou et al.90
The trunk bark and seeds of this plant are boiled together with Carica papaya leaves, the seeds of Aframomum melegueta and lime and used for treatment of malaria and other fevers.seeds83, 84 and 85Antiplasmodial activity, Ngemenya et al.91
Leguminosae- MimosoideaeCylicodiscus gabunensisUsed to prepare remedies for infectious diseases and is known for its antibacterial and antiplasmodial activities.stem bark86Exhibited antimicrobial activity against Proteus vulgaris, Nchancho et al.92
Albizia adianthifoliaUsed traditionally to treat several ailments, including infectious and associated diseases.stem bark24Antioxidant and antimicrobial activities, Tamokou et al.93
MonimiaceaeGlossocalyx brevipesThe macerated leaves are added to anti-fever preparations.leaves87, 88 and 89Anti-malarial, Mbah et al.66
MoraceaeMorus mesozygiaRoots, stem and leaves are used to treat syphilis, dermatitis, rheumatism, asthenias, fever and malaria.stem bark90, 91 and 92Cytotoxic and anti-malarial, Zelefack et al.68
Antiaris africanaBark extracts are used for the treatment of chest pain, leaf decoctions for the treatment of syphilis, and the latex is a purgative agent. It is also used in the treatment of sore throat, leprosy and cancer.stem bark93Antioxidant and anticancer, Kuete et al.94
Treculia obovoideaTraditionally used to treat skin diseases, dental allergy, amoebic dysentery and AIDStwigs94Antimicrobial activity, Kuete et al.95
MoraceaeArtocarpus communisTreatment of cardiovascular diseases, used as food; other parts of the plants are traditionally used to treat headache, infectious and associated diseases such as toothache, eye problems, ear infections, herpes, enlarged spleen, sprains, contusions, swelling, chest pain and vomiting from heart problems, boils, abscess, and skin infections.root95 and 96Antimicrobial activities, Kuete et al.96
Dorstenia barteriUsed in the treatment of malaria.twigs97, 98, 99, 100, 101 and 102Anti-malarial activity, Ngameni et al.69
Ficus cordataUsed against hyperaesthesia, ataxia, muscle tremor, padding motions and jaundice, which could be a symptom of several related liver diseases.stem bark11, 103, 104, 105 and 106Hepatoprotective and cytotoxic, Donfack et al.97
MyristicaceaePycnanthus angolensisTreatment of stomach pain, chest pain and rhinitis problems, malaria, toothache, fungal skin infections, chest pain, oral thrush, and worms; some further claim a folkloric use for the treatment of leprosy.stem bark107, 108 and 109Antifungal activity, Wabo et al.98
OlacaceaeCoula edulisTreatment of stomach ache and skin diseases.stem bark110 and 111Antidermatophytic activity against Microsporum audouinii and Epidermophyton floccoseum, Tamokou et al.99
SapotaceaeBaillonella ToxispermaTreatment of abscesses, infertility, stomach troubles, convulsion, rheumatism and malaria.stem bark49 and 112Activity against Plasmodium falciparum, Mbah et al.70
VerbenaceaeVitex cienkowskiiUsed in the treatment of many disorders, including cardiovascular disease.stem bark113Vasorelaxant, antioxidant and hypertensive effect, Dongmo et al.100
ZingiberaceaeAframomum zambesiacumUsed to treat fevers.seeds114, 115 and 116Anti-malarial, Kenmogne et al.71
Reneilmia cincinnataUsed to treat fevers and as a spice.fruits117, 118 and 119Anti-malarial, Tchuendem et al.72

Figure 8

Chemical structures of bioactive metabolites IV. Compounds 107 to 119

Figure 8 Chemical structures of bioactive metabolites IV. Compounds 107 to 119.

The isoflavonoids 3’,4’-dihydroxy-7-O-[(E)-3,7-dimethyl-2,6-octadienyl]isoflavone (75) and Griffonianone C (76), which have been isolated from Millettia griffoniana (used traditionally to treat female sterility, amenorrhea and menopausal disoders), both showed interesting estrogenic activities, thus validating the ethnobotanical uses of the plant45. Meanwhile, the anticancer properties of 3,3’-dimethoxy-4’-O-β-D-xylopyronosylellagic acid (93) from Antiaris africana (a plant traditionally used to treat cancer, amongst other ailments), has been verified. This compound indicated a pronounced tumor reducing activity (96.64%) in potato disk tumor induction assay and also showed interesting inhibitory properties against human DU-145 and hepatocarcinoma Hep G2 cells with > 70% inhibition at 50 μg/mL94. At the same time, the ceramide tanacetamide (113), with an interesting vasorelaxant property, was isolated along with salvin A and maslinic acid from Vitex cienkowskii, thus validating the use of the plant in the treatment of cardiovascular diseases100. The antidiabetic property of Terminalia superba has been verified by the isolation of 3,4’-di-O-methylellagic acid 3’-O-β-D-xylopyranoside (45) and 4’-O-galloy-3,3’-di-O-methylellagic acid 4-O-β-D-xylopyrano-side (46), which both exhibited good α-glucosidase inhibition80, while the antioxidant properties of the compounds isolated from Symphonia globulifera could also be used to validate the use of the plant in the preparation of tonics for pregnant women65.
Among the plants used in the treatment of diseases related to the liver, the most promising are Ficus cordata and Irvingia gabonensis, from which compounds with hepatoprotective properties have been isolated86, 97. Among the isolated compounds, 3-friedelanone (53) might be useful for the prevention of toxin-induced and free radical-mediated liver diseases86.
Plants used for the treatment of microbial infections have also been heavily explored. Compounds with antisalmonellal activity have been isolated from plants which have been traditionally used to treat typoid fever (an infection principally caused by Salmonella typhi and related baccili). The plant species include: Turraeanthus africanus, from which 12,15-epoxylabda-8(17),12,14-trien-16-acetate (82) has been isolated and was found to be the only active principle, possessing the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of respectively 25 μg/mL and 100 μg/mL against Salmonella typhi, S. paratyphi A and S. paratyphi B90; and Cassia petersiana from which stigmasterol-3-O-β-D-glucoside (35) was identified as the active principle77. Several other plant species have been investigated for their potential for antimicrobial agents due to their uses in traditional medicine. The isolated metabolites have been tested against a wide range of Gram-positive bacteria, Gram-negative bacteria and fungi. The most convincing studies for antifungal plants were carried out by Ndom et al.75, Ngounou et al.76, Dzoyem et al.81, Tsaffack et al.83, Wabo et al.98 and Tamokou et al.99 on Senecio mannii, Ceiba pentandra, Diospyros canaliculata, Psorospermum febrifugum, Pycnanthus angolensis and Coula edulis respectively. Besides, the diterpenoid hardwickiic acid (74), which was isolated from Irvingia gabonensis (used in the treatment of gonorrhoea, among other ailments) was shown to be particularly active against Neisseria gonorrhoeae in vitro with an IC50 of 4.26 μM85.


In this paper, an attempt has been made to establish trends towards a chemotaxonomic classification of medicinal plants from Cameroon (located in tropical Africa) and to document dispersed data on plants, whose ethnobotanical uses have been validated by bioassay screening of the isolated phytochmicals. The information contained herein could serve as a starting point for further studies on Cameroonian medicinal plants. This further demonstrates that the Cameroon rainforest has a great potential for new drugs and improved plant medicines15, 19. However, plants from a good number of families have never been investigated phytochemically. These include, among others, the Agavadeae, Amaranthaceae, Araceae, Aspidiaceae, Bamingtoniaceae, Basellaceae, Begoniaceae, Capparaceae, Caricaceae, Chenopodiaceae, Commelinaceae, Convolvulaceae, Cuburbitaceae, Ericaceae, Icacinaceae, Lecydiaceae, Loranthaceae, Lythraceae, Malvaceae, Musaceae, Nyctginaceae and Oxalicaceae, which have diverse uses in traditional medicine20. Moreover, a majority of the plant species studied have been harvested in the rainforests of the Southern part of the country (our statistacs show that > 95% of the isolated metabolites were derived from plants harvested in the North West, South West, Littoral and Western plateau as well as the Centre, South and East regions). However, on the basis of the fact that the Northern parts of the country represent the semi-arid and arid regions, the variations in soil composition and climatic conditions could result in dramatic variations of phytochemical compositions of the plant species and should be subject to further investigation, since the local populations in this part of the country are heavily dependent on traditional herbal medicines for their primary health care. One species which needs to be urgently subjected to phytochemical investigation is Bridelia ferruginea, whose leaves, barks and fruits are used for the treatment of dysentery, diabetes and as a remedy for thrush (mycotic stomatitis) in children101, 102, and as an antidote for snake bite103. In addition, the root decoction is also used for the treatment of gonorrhoea104 and as an antidote for poisons103. The ethnobotanical use of this plant has been validated by in vitro activity of the leaf extracts against Pseudomonas frutescens, Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, and Echerichia coli103. Another species that requires to be investigated is Piper guineense, whose antifungal properties have been verified by screening of the leaf extracts105. Moreover, the phytochemical contents of 26 species that are currently used for the treatment of hypertension and cardiovascular problems in the Bafia tribal people27, could be further investigated with a view to identifying potential lead compounds for the development of drugs against cardiac problems, while those used to treat intestinal disorders in Mbalmayo, Central region106, could also be examined for their antimicrobial potential.

Authors’ information

WS and SMNE are professors of medicinal chemistry generally interested in drug design and discovery, while SMNE also focuses organic synthesis and on natural product leads from Cameroonian medicinal plants. LMM is an associate professor of organic chemistry actively involved in the isolation and characterization of secondary metabolites from Cameroonian medicinal plants. LLL holds a PhD in environmental science and manages a Chemical and Bioactivity Information Centre (CBIC) with a focus on developing databases for information from medicinal herbs in Africa. PNJ is a retired research officer of Lhasa Ltd who currently leads the CBIC branch in Leeds, UK. FNK is a PhD student working on CADD under the joint supervision of LCOO and EM, while NE is a medical practitioner engaged in a comparative study of WM and ATM.


African traditional medicine
highthroughput screening
Research and Development
World Health Organization
Western medicine.



Financial support for this work is acknowledged from Lhasa Ltd. Mr Jean Alexandre Lado (Department of Chemistry, University of Yaoundé I) and Mr Serge Alain Foboufou Tanemossou (Leibnitz Institute of Plant Biochemistry, Halle, Germany) are acknowledged for their support, while Dr James Mbah (Department of Chemistry, University of Buea) is acknowledged for constructive discussions. Drs Denis Zofou and Moses Ngemenya (Department of Biochemistry, University of Buea) also are acknowledged for proofreading and criticising the draft manuscript.

Electronic supplementary material

Additional file 1: Full list of consulted journals in constructing CamMedNP.(DOC 34 KB)

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1
Authors’ original file for figure 2
Authors’ original file for figure 3
Authors’ original file for figure 4
Authors’ original file for figure 5
Authors’ original file for figure 6
Authors’ original file for figure 7
Authors’ original file for figure 8

Competing interests

The authors declare no conflicts of interest.

Authors’ contributions

WS, LMM, and SMNE conceived the idea. FNK, NE, LCOO, and EM participated in the data collection. All authors contributed in the data analysis, the discussion of results and the conception of the paper. FNK wrote the first draft of the paper and all authors agreed on the final version before submission.


    1. Hostettmann K, Marston A, Ndjoko K, Wolfender JL: The potential of African plants as a source of drugs. Curr Org Chem. 2000, 4: 973-1010. 10.2174/1385272003375923.
    2. Vasisht K, Kumar V: Compendium of Medicinal and Aromatic Plants, vol. 1. 2004, Africa: ICS-UNIDO, Trieste
    3. Efange SMN: Natural products: a continuing source of inspiration for the medicinal chemist. Advances in Phytomedicine. Edited by: Iwu MM, Wootton JC. 2002, Amsterdam: Elsevier Science, 61-69.
    4. World Health Organisation: Traditional medicine. Fact sheet No 134. 2003, Geneva: WHO
    5. World Health Organisation: WHO traditional medicine strategy: 2002–2005. 2001, Geneva: WHO
    6. Ngono Ngane RA, Koanga Mogtomo ML, Tchinda Tiabou A, Magnifouet Nana H, Motso Chieffo PR, Mballa Bounou Z, Ebelle Etame RM, Ndifor F, Biyiti L, Amvam Zollo PH: Ethnobotanical survey of some Cameroonian plants used for treatment of viral diseases. Afr J Plant Sci. 2001, 5 (1): 15-21.
    7. Cunningham AB: African medicinal plant: setting priorities at the interface between conservation and primary health care. People and plants working paper 1. 1993, Paris: UNESCO
    8. Chin YW, Balunas MJ, Chai HB, Kinghorn AD: Drug discovery from natural sources. AAPS J. 2006, 8 (2): E239-E253.
    9. Li JWH, Vederas JC: Drug discovery and natural products: end of an era or an endless frontier?. Science. 2009, 325: 161-165. 10.1126/science.1168243.
    10. Butler MS: Natural products to drugs: natural product derived compounds in clinical trials. Nat Prod Rep. 2005, 22: 162-195. 10.1039/b402985m.
    11. Holmstedt BR, Bruhn JG: Ethnopharmacology – a challenge. Ethnobotany, Evolution of a Discipline. Edited by: Schultes RE, Von Reis S. 1995, Portland, Oregon: Dioscorides Press, 338-342.
    12. World Health Organisation: The promotion and development of traditional medicine. Technical representative series. 1978, Geneva: WHO
    13. Cunningham AB: An investigation of the herbal medicine trade in Natal/KwaZulu. Investigational Report no. 29. 1988, Institute of Natural Resources, University of Natal: Pietermaritzburg
    14. World Health Organisation: World health statistics 2009: Cause-specific mortality and morbidity. 2009, Geneva: WHO
    15. Kuete V, Efferth T: Cameroonian medicinal plants: pharmacology and derived natural products. Front Pharmacol. 2010, 1: 123-
    16. Nkongmeneck BA, Mapongmetsem PM, Pinta YV, Nkuinkeu R, Tsabang N, Fongnzossie E, Kemeuze V, Jiofack T, Johnson M, Asaha S, Sakwe C, Mboufack C: Etat des lieux des plantes médicinales importantes à conserver et des jardins des plantes médicinales à promouvoir. 2007, Geneva: Rapport CEN/OMS/MEM
    17. Mbatchou GPT: Plant diversity in Central African rain forest: Implications for biodiversity and conservation in Cameroon. 2004, Wageningen University, Department of Plant Sciences: PhD thesis
    18. Earth Trends: Evolution of Cameroon Protected Areas (1995 – 2008). 2003, Washington DC: World Resource Institute
    19. Kuete V: Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med. 2010, 76: 1479-1491. 10.1055/s-0030-1250027.
    20. Adjanohoun JE, Aboubakar N, Dramane K, Ebot ME, Ekpere JA, Enow-Orock EG, Focho D, Gbile ZO, Kamanyi A, Kamsu Kom J, Keita A, Mbenkum T, Mbi CN, Mbiele AL, Mbome IL, Mubiru NK, Nancy WL, Nkongmeneck B, Satabie B, Sofowora A, Tamze V, Wirmum CK: Traditional medicine and pharmacopoeia: contribution to ethnobotanical and floristic studies in Cameroon. 1996, Addis-Ababa: OAU/STRC
    21. Jiofack T, Fokunang C, Kemeuze V, Fongnzossie E, Nkongmeneck BA, Mapongmetsem PM, Tsabang N: Ethnobotanical uses of medicinal plants of two ethnoecological regions of Cameroon. Int J Med Sci. 2010, 2: 60-79.
    22. Jiofack T, Fokunang C, Guedge N, Kemeuze V, Fongnzossie E, Nkongmeneck BA, Mapongmetsem PM, Tsabang N: Ethnobotanical uses of some plants of two ethnoecological regions of Cameroon. Afr J Pharm Pharmacol. 2009, 3: 664-684.
    23. Jiofack T, Fokunang C, Kemeuze V, Fongnzossie E, Tsabang N, Nkuinkeu R, Mapongmetsem PM, Nkongmeneck BA: Ethnobotany and phytopharmacopoea of the South- West ethnoecological region of Cameroon. J Med Plant Res. 2008, 2: 197-206.
    24. Jiofack T, Ayissi I, Fokunang C, Guedge N, Kemeuze V: Ethnobotany and phytomedicine of the upper Nyong valley forest in Cameroon. Afr J Pharm Pharmacol. 2009, 3: 144-150.
    25. Simbo DJ: An ethnobotanical survey of medicinal plants in Babungo, Northwest Region, Cameroon. J. Ethnobiol Ethnomed. 2010, 6: 8-10.1186/1746-4269-6-8.
    26. Focho DA, Newuh MC, Anjah MG, Nwana FA, Ambo FB: Ethnobotanical survey of trees in Fundong, Northwest Region, Cameroon. J Ethnobiol Ethnomed. 2009, 5: 17-10.1186/1746-4269-5-17.
    27. Noumi E, Houngue F, Lontsi D: Traditional medicines in primary health care: plants used for the treatment of hypertension in Bafia, Cameroon. Fitoterapia. 1999, 70: 134-139. 10.1016/S0367-326X(98)00025-2.
    28. Noumi E, Ebwelle ES: Potentiality of medicinal plants in treating urinary lithiasis in Littoral Region, Cameroon. Eur J Med Plant. 2011, 1 (3): 74-87.
    29. Noumi E: Ethno-medico-botanical survey of medicinal plants used in the treatment of asthma in the Nkongsamba Region, Cameroon. Ind J Trad Knowledge. 2010, 9 (3): 491-495.
    30. Noumi E, Dibakto TW: Medicinal plants used for peptic ulcer in the Bangangte region, Western Cameroon. Fitoterapia. 2000, 17 (4): 406-412.
    31. Noumi E, Eloumou MER: Syphilis ailment: Prevalence and herbal remedies in Ebolowa subdivision (South region, Cameroon). Int J Pharm Biomed Sci. 2011, 2 (1): 20-28.
    32. Mpondo EM, Dibong SD: Traditional knowledge on medicinal plants use by ethnic communities in Douala, Cameroon. Eur J Med Plant. 2012, 2 (2): 159-176.
    33. Mpondo EM, Dibong SD: Medicinal plant knowledge of ethnic groups in Douala town, Cameroon. Am J Food Nut. 2011, 1 (4): 174-184.
    34. Din N, Dibong SD, Mpondo EM, Priso RJ, Kwin NF, Ngoye A: Inventory and Identification of Plants Used in the Treatment of Diabetes in Douala Town (Cameroon). Eur J Med Plant. 2011, 1 (3): 60-73.
    35. Dibong SD, Mpondo EM, Ngoye A, Kwin NF, Betti JL: Ethnobotanique et phytomédecine des plantes médicinales vendues sur les marchés de Douala, Cameroun. J App Biosci. 2011, 37: 2496-2507.
    36. Dibong SD, Mpondo EM, Ngoye A, Priso RJ: Modalities of exploitation of medicinal plants in Douala region. Am J Food Nut. 2011, 1 (2): 67-73. 10.5251/abjna.2011.
    37. Dibong SD, Mpondo EM, Ngoye A, Kwin NF: Plantes médicinales utilisées par les populations bassa de la région de Douala au Cameroun. Int J Biol Chem Sci. 2011, 5 (3): 1105-1117.
    38. Dibong SD, Mpondo EM, Ngoye A, Priso RJ: Inventory and biodiversity of species edible wild fruits sold in the markets of Douala, Cameroon. Int J App Biol Pharm Tech. 2011, 2 (3): 303-311.
    39. Ngo Bum E, Taiwe GS, Moto FCO, Ngoupaye GT, Vougat RRN, Sakoue VD, Gwa C, Ayissi ER, Dong C, Rakotonirina A, Rakotonirina SV: Antiepileptic medicinal plants used in traditional medicine to treat epilepsy. Clinical and Genetic Aspects of Epilepsy. Edited by: Afawi Z. 2011, Rijeka: InTech, 175-192.
    40. Betti JL: An ethnobotanical study of medicinal plants among the Baka Pygmies in the Dja Biosphere reserve, Cameroon. Afr Study Monographs. 2004, 25 (1): 1-27.
    41. Sandberg F, Perera-Ivarsson P, El-Seedi HR: A Swedish collection of medicinal plants from Cameroon. J Ethnopharmacol. 2005, 102 (3): 336-343. 10.1016/j.jep.2005.06.032.
    42. Titanji VPK, Zofou D, Ngemenya M: The antimalarial potential of medicinal plants used for the treatment of malaria in Cameroonian folk medicine. Afr J Trad CAM. 2008, 5 (3): 302-321.
    43. Ntie-Kang F, Mbah JA, Mbaze LM, Lifongo LL, Scharfe M, Ngo Hanna J, Cho-Ngwa F, Amoa Onguéné P, Owono Owono LC, Megnassan E, Sippl W, Efange SMN: CamMedNP: Building the Cameroonian 3D Structural Natural Products Database for Virtual Screening. BMC Complement Altern Med. 2013, 13: 88-10.1186/1472-6882-13-88.
    44. Lipinski CA, Lombardo F, Dominy BW, Feeney PJ: Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv Drug Delivery Rev. 1997, 23: 3-25. 10.1016/S0169-409X(96)00423-1.
    45. Wanda GJMK, Njamen D, Yankep E, Fotso MT, Fomum ZT, Wober J, Starcke S, Zierau O, Vollmer G: Estrogenic properties of isoflavones derived from Millettia griffoniana. Phytomedicine. 2006, 13: 139-145. 10.1016/j.phymed.2005.06.003.
    46. Kamdem STR, Wafo P, Ali Z, Oluyemisi OO, Marasini BP, Khan IA, Ngadjui BT, Choudhary MI, Rahman AU: Chemical constituents of Stereospermum acuminatissimum and their urease and α-chymotrypsin inhibitions. Fitoterapia. 2012, 83: 204-208. 10.1016/j.fitote.2011.10.014.
    47. Sob SVT, Wabo HK, Tang CT, Tane P, Ngadjui BT, Ye Y: Phenol esters and other constituents from the stem barks of Stereospermum acuminatissimum. J Asian Nat Prod Res. 2011, 13: 1128-1134. 10.1080/10286020.2011.619182.
    48. Lenta BN, Weniger B, Antheaume C, Noungoue DT, Ngouela S, Assob JCN, Vonthron-Senecheau C, Fokou PA, Devkota KP, Tsamo E, Sewald N: Anthraquinones from the stem bark of Stereospermum zenkeri with antimicrobial activity. Phytochemistry. 2007, 68: 1595-1599. 10.1016/j.phytochem.2007.03.037.
    49. Tala MF, Krohn K, Hussain H, Kouam SF, Wabo HK, Tane P, Schulz B, Hu Q: Laurentixanthone C: A new antifungal and algicidal xanthone from stem bark of Vismia laurentii. Z. Naturforsch. 2007, 62b: 565-568.
    50. Poumale HMP, Amadou D, Shiono Y, Kapche GDWF, Ngadjui BT: Chemical constituents of Dorstenia convexa (Moraceae). Asian J Chem. 2011, 23 (2): 525-527.
    51. Vouffo B, Hussain H, Eyong KO, Dongo E, Folefoc GN, Nkengfack AE, Krohn K: Chemical constituents of Dorstenia picta and Newbouldia laevis. Biochem Syst Ecol. 2008, 36: 730-732. 10.1016/j.bse.2008.06.004.
    52. Metuno R, Ngandeu F, Tchinda AT, Ngameni B, Kapche GDWF, Djemgou PC, Ngadjui BT, Bezabih M, Abegaz BM: Chemical constituents of Treculia acuminata and Treculia africana (Moraceae). Biochem Syst Ecol. 2008, 36: 148-152. 10.1016/j.bse.2007.06.012.
    53. Vouffo B, Krohn K, Kouam SF, Hussain H, Dongo E, Meier K, Schulz B: Dinklagenonoate: A new isobauerane-type triterpenoid and other minor constituents from the twigs of Dorstenia dinklagei. Biochem Syst Ecol. 2008, 36: 655-658. 10.1016/j.bse.2008.03.014.
    54. Zintchem AA, Bikobo DN, Atchadé AT, Ngo Mbing J, Gangoue-Pieboji J, Tih RG, Blond A, Pegnyemb DE, Bodo B: Nitrile glucosides and serotobenine from Campylospermum glaucum and Ouratea turnarea. Phytochemistry. 2008, 69: 2209-2213. 10.1016/j.phytochem.2008.04.013.
    55. Zintchem AA, Atchadé AT, Tih RG, Mbafor JT, Blond A, Pegnyemb DE, Bodo B: Flavonoids from Ouratea staudtii Van Tiegh. (ex Keay) (Ochnaceae). Biochem Syst Ecol. 2007, 35: 255-256. 10.1016/j.bse.2006.10.018.
    56. Tih EA, Ghogomu RT, Sondengam BL, Caux C, Bodo B: Constituents of Lophira alata leaves. Biochem Syst Ecol. 2003, 31: 549-551. 10.1016/S0305-1978(02)00187-4.
    57. Happi EN, Kamdem Wafo AF, Wansi JD, Ngadjui BT, Sewald N: O-prenylated acridone alkaloids from the stems of Balsamocitrus paniculata (Rutaceae). Planta Med. 2011, 77: 934-938. 10.1055/s-0030-1250692.
    58. Ngadjui BT, Ayafor JF, Sondengam BL, Connolly JD: Limonoids from Clausena anisata. J Nat Prod. 1989, 52: 832-836. 10.1021/np50064a027.
    59. Ayimele GA, Tane P, Connolly JD: Aulacocarpin A and B, nerolidol and β-sitosterol glucoside from Aframomum escapum. Biochem Syst Ecol. 2004, 32: 1205-1207. 10.1016/j.bse.2004.03.012.
    60. Wabo HK, Tane P, Connolly JD: Diterpenoids and sesquiterpenoids from Aframomum arundinaceum. Biochem Syst Ecol. 2006, 34: 603-605. 10.1016/j.bse.2006.02.001.
    61. Balick MJ, Cox PA: Plants, People, and Culture: The Science of Ethnobotany. 1996, New York: Scientific American Library
    62. Zofou D, Kowa TK, Wabo HK, Ngemenya MN, Tane P, Titanji VPK: Hypericum lanceolatum (Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark. Malar J. 2011, 10: 167-10.1186/1475-2875-10-167.
    63. Lenta BN, Ngouela S, Boyom FF, Tantangmo F, Tchouya GRF, Tsamo E, Gut J, Rosenthal PJ, Connolly JD: Anti-plasmodial activity of some constituents of the root bark of Harungana madagascariensis LAM. (Hypericaceae. Chem Pharm Bull. 2007, 55 (3): 464-467. 10.1248/cpb.55.464.
    64. Lannang AM, Louh GN, Lontsi D, Specht S, Sarite SR, Flörke U, Hussain H, Hoerauf A, Krohn K: Antimalarial compounds from the root bark of Garcinia polyantha Olv. J Antibiot. 2008, 61 (8): 518-523. 10.1038/ja.2008.70.
    65. Ngouela S, Lenta BN, Noungoue DT, Ngoupayo J, Boyom FF, Tsamo E, Gut J, Rosenthal PJ, Connolly JD: Anti-plasmodial and antioxidant activities of constituents of the seed shells of Symphonia globulifera Linn f. Phytochemistry. 2006, 67: 302-306. 10.1016/j.phytochem.2005.11.004.
    66. Mbah JA, Tane P, Ngadjui BT, Connolly JD, Okunji CC, Iwu MM, Schuster BM: Antiplasmodial agents from the leaves of Glossocalyx brevipes. Planta Med. 2004, 70: 437-440.
    67. Bickii J, Tchouya GRF, Tchouankeu JC, Tsamo E: The antiplasmodial agents of the stem bark of Entandrophragma angolense (Meliaceae). Afr J Trad CAM. 2007, 4 (2): 135-139.
    68. Zelefack F, Guilet D, Valentin A, Fongang RCS, Kom B, Chevalley S, Ngouela SA, Tsamo E, Fabre N, Dijoux-Franca MG: Antiplasmodial and cytotoxic activities of flavonoids and arylbenzofuran derivatives from Morus mesozygia. Greener J Biol Sci. 2012, 2 (2): 20-24.
    69. Ngameni B, Wantchueng J, Boyom FF, Keumedjio F, Ngadjui BT, Gut J, Abegaz BM, Rosenthal PJ: Antimalarial prenylated chalcones from the twigs of Dorstenia barteri var. subtriangularis. Arkivoc. 2007, xiii: 116-123.
    70. Mbah JA, Ndikum G, Zofou D, Ngemenya MN, Efange SMN: Antiplasmodial triterpenes from the stem bark of Baillonella toxisperma. ISESCO J Sci Tech. 2011, 7 (12): 84-87.
    71. Kenmogne M, Prost E, Harakat D, Jacquier MJ, Frédérich M, Sondengam LB, Zèches M, Waffo-Téguo P: Five labdane diterpenoids from the seeds of Aframomum zambesiacum. Phytochemistry. 2006, 67: 433-438. 10.1016/j.phytochem.2005.10.015.
    72. Tchuendem MHK, Mbah JA, Tsopmo A, Ayafor JF, Sterner O, Okunji CC, Iwu MM, Schuster BM: Anti-plasmodial sesquiterpenoids from the African Reneilmia cincinnata. Phytochemistry. 1999, 52: 1095-1099. 10.1016/S0031-9422(99)00344-1.
    73. Tamokou JD, Kuiate JR, Tene M, Nwemeguela TJK, Tane P: The antimicrobial activities of extract and compounds isolated from Brillantaisia lamium. Iran J Med Sci. 2011, 36 (1): 24-31.
    74. Nkanwen ERS, Gatsing D, Ngamga D, Fodouop SPC, Tane P: Antibacterial agents from the leaves of Crinum purpurascens herb (Amaryllidaceae). Afr Health Sci. 2009, 9 (4): 264-269.
    75. Ndom JC, Mbafor JT, Kakam Z, Happi N, Vardamides JC, Mbaze Meva’a L, Mpondo TN, Fomum ZT: Styrylpyrone glucosides with antimicrobial activity from Senecio mannii Hook. (Asteraceae). Bol Latinoam Caribe Plant Med Aromaticas. 2007, 6 (3): 73-80.
    76. Ngounou FN, Meli AL, Lontsi D, Sondengam BL, Rahman AU, Choudhary MI, Malik S, Akhtar F: New isoflavones from Ceiba pentandra. Phytochemistry. 2000, 54: 107-110. 10.1016/S0031-9422(00)00035-2.
    77. Djemgou PC, Gatsing D, Kenmogne M, Ngamga D, Aliyu R, Adebayo AH, Tane P, Ngadjui BT, Seguin E, Adoga GI: Antisalmonella agent and a new dihydroanthracenone from Cassia petersiana. Res J Med Plant. 2007, 1 (2): 65-71.
    78. Gatsing D, Djemgou PC, Garba IH, Aliyu R, Tchuendem MH, Tane P, Ngadjui BT, Seguin E, Adoga GI: Dihydronaphthalenone and chromone from Cassia petersiana Bolle and the antisalmonellal activity of its crude extract. Res J Phytochem. 2010, 4 (3): 201-206.
    79. Rahman AU, Ngounou FN, Choudhary MI, Malik S, Makhmoor T, Nur-E-Alam M, Zareen S, Lontsi D, Ayafor JF, Sondengam BL: New antioxidant and antimicrobial ellagic acid derivatives from Pteleopsis hylodendron. Planta Med. 2001, 67: 335-339. 10.1055/s-2001-14306.
    80. Tabopda TK, Ngoupayo J, Liu J, Ali MS, Khan SN, Ngadjui BT, Luu B: α-Glucosidase inhibitors ellagic acid derivatives with immunoinhibitory properties from Terminalia superba. Chem Pharm Bull. 2008, 56 (6): 847-850. 10.1248/cpb.56.847.
    81. Dzoyem JP, Kechia FA, Kuete V, Pieme AC, Akak CM, Tangmouo JG, Lohoue PJ: Phytotoxic, antifungal activities and acute toxicity studies of the crude extract and compounds from Diospyros canaliculata. Nat Prod Res. 2011, 25 (11): 741-749.
    82. Azebaze AGB, Ouahouo BMW, Vardamides JC, Valentin A, Kuete V, Acebey L, Beng VP, Nkengfack AE, Meyer M: Antimicrobial and antileishmanial xanthones from the stem bark of Allanblackia gabonensis. Chem Nat Compd. 2008, 44 (5): 582-587. 10.1007/s10600-008-9141-9.
    83. Tsaffack M, Nguemeving JR, Kuete V, Tchize BSN, Mkounga P, Beng VP, Hultin PG, Tsamo E, Nkengfack AE: Two new antimicrobial dimeric compounds: febrifuquinone, a vismione-anthraquinone coupled pigment and adamabianthrone, from two Psorospermum species. Chem Pharm Bull. 2009, 57 (10): 1113-1118. 10.1248/cpb.57.1113.
    84. Lenta BN, Kamdem LM, Ngouela S, Tantangmo F, Devkota KP, Boyom FF, Rosenthal PJ, Tsamo E: Antiplasmodial constituents from the fruit pericarp of Pentadesma butyracea. Planta Med. 2011, 77: 377-379. 10.1055/s-0030-1250384.
    85. Kuete V, Wabo GF, Ngameni B, Mbaveng AT, Metuno R, Etoa FX, Ngadjui BT, Beng VP, Meyer JJM, Lall N: Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvingia gabonensis (Ixonanthaceae). J Ethnopharmacol. 2007, 114 (1): 54-60. 10.1016/j.jep.2007.07.025.
    86. Donfack JH, Fotso GW, Ngameni B, Tsofack FN, Tchoukoua A, Ambassa P, Abia W, Tchana AN, Giardina S, Buonocore D, Vita Finzi P, Vidari G, Marzatico F, Ngadjui BT, Moundipa PF: In vitro hepatoprotective and antioxidant activities of the crude extract and isolated compounds from Irvingia gabonensis. Asian J Trad Med. 2010, 5 (3): 79-88.
    87. Kouam J, Siewe Noundou X, Mabeku LBK, Lannang AM, Choudhary MI, Fomum ZT: Sigmoiside E: A new antibacterial triterpenoid saponin from Erythrina sigmoidea (Hua). Bull Chem Soc Ethiop. 2007, 21 (3): 373-378.
    88. Kouam J, Etoa FX, Mabeku LBK, Fomum ZT: Sigmoidine L, a new antibacterial flavonoid from Erythrina sigmoidea (Fabaceae). Nat Prod Comm. 2007, 2 (11): 1105-1108.
    89. Kouam J, Meli AL, Kuete V, Fomum ZT: Flavanones from the stem bark of Erythrina sigmoidea. Nat Prod Comm. 2008, 3 (11): 1895-1898.
    90. Djemgou PC, Gatsing D, Hegazy MEF, Mohamed AEH, Ngandeu F, Tane P, Ngadjui BT, Fotso S, Laatsch H: Turrealabdane, turreanone and an antisalmonellal agent from Turraeanthus africanus. Planta Med. 2010, 76: 165-171. 10.1055/s-0029-1186038.
    91. Ngemenya MN, Akam TM, Yong JN, Tane P, Fanso-Free SNY, Berzins K, Titanji VPK: Antiplasmodial activities of some products from Turreanthus africanus (Meliaceae). Afr J Health Sci. 2006, 13: 33-39.
    92. Nchancho K, Kouam J, Tane P, Kuete V, Wantchueng J, Fomum ZT: Coumestan glycosides from the stem bark of Cylicodiscus gabunensis. Nat Prod Comm. 2009, 4 (7): 931-934.
    93. Tamokou JD, Mpetga DJS, Lunga PK, Tene M, Kuiate JR: Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae). BMC Complement Altern Med. 2012, 12: 99-10.1186/1472-6882-12-99.
    94. Kuete V, Vouffo B, Mbaveng AT, Vouffo EY, Siagat RM, Dongo E: Evaluation of Antiaris africana methanol extract and compounds for antioxidant and antitumor activities. Pharm Biol. 2009, 47 (11): 1042-1049. 10.3109/13880200902988595.
    95. Kuete V, Metuno R, Ngameni B, Mbaveng AT, Ngandeu F, Fotso GW, Bezabih M, Etoa FX, Ngadjui BT, Abegaz BM, Beng VP: Antimicrobial activity of the methanolic extracts and compounds from Treculia obovoidea (Moraceae). J Ethnopharmacol. 2007, 112 (3): 531-536. 10.1016/j.jep.2007.04.010.
    96. Kuete V, Ango PY, Fotso GW, Kapche GDWF, Dzoyem JP, Wouking AG, Ngadjui BT, Abegaz BM: Antimicrobial activities of the methanol extract and compounds from Artocarpus communis (Moraceae). BMC Complement Altern Med. 2011, 11: 42-10.1186/1472-6882-11-42.
    97. Donfack HJ, Kengap RT, Ngameni B, Chuisseu PDD, Tchana AN, Buonocore D, Ngadjui BT, Moundipa PF, Marzatico F: Ficus cordata Thunb (Moraceae) is a potential source of some hepatoprotective and antioxidant compounds. Pharmacologia. 2011, 2: 137-145.
    98. Wabo HK, Tatsimo SN, Tane P, Connolly JD: Pycnathuquinone C: A new terpenoid-quinone from Pycnathus angolensis. Planta Med. 2007, 73: 187-189. 10.1055/s-2007-967103.
    99. Tamokou JD, Kuiate JJ, Gatsing D, Nkeng-Efouet AP, Njouendou AJ: Antidermatophytic and toxicological evaluations of dichloromethane-methanol extract, fractions and compounds isolated from Coula edulis. Iran J Med Sci. 2011, 36 (2): 111-121.
    100. Dongmo AB, Azebaze AGB, Donfack FM, Dimo T, Nkeng-Efouet PA, Davkota KP, Sontia B, Wagner H, Sewald N, Vierling W: Pentacyclic triterpenoids and ceramide mediate the vasorelaxant activity of Vitex cienkowskii via involvement of NO/cGMP pathway in isolated rat aortic rings. J Ethnopharmacol. 2011, 113: 204-212.
    101. Talla E, Djamen D, Djouldé DR, Tatsadjeu L, Tantoh D, Mbafor JT, Fomum ZT: Antimicrobial activity of Bridelia ferruginea leaves extracts. Fitoterapia. 2002, 73: 343-345. 10.1016/S0367-326X(02)00084-9.
    102. Dalziel JM: The useful plants of west Tropical Africa. 1937, London: Crown Agents for the Colonies
    103. Hentchoya HJ: Contribution à l’etude de plantes medecinales du Cameroun. 1991, Ngaoundéré: Université de Ngaoundéré, Rapport sur la prospection et la sélection de quelques plantes devant faire l’objet d’une étude chimique
    104. Irobi ON, Moo-Young M, Anderson WA, Daramola SO: Antimicrobial activity of bark extracts of Bridelia ferruginea (Euphorbiaceae). J Ethnopharmacol. 1994, 43: 185-190. 10.1016/0378-8741(94)90041-8.
    105. Ngono Ngane A, Biyiti L, Bouchet P, Nkengfack A, Amzam Zollo PH: Antifungal activity of Piper guineense of Cameroon. Fitoterapia. 2003, 74: 464-468. 10.1016/S0367-326X(03)00112-6.
    106. Noumi E, Yomi A: Medicinal plants used for intestinal diseases in Mbalmayo region, Central Province, Cameroon. Fitoterapia. 2001, 72: 246-254. 10.1016/S0367-326X(00)00288-4.

Pre-publication history

  1. The pre-publication history for this paper can be accessed here:


© Ntie-Kang et al.; licensee BioMed Central Ltd. 2013
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Author: Eco Supplements - About Us

Editors of Eco Supplements portal
We are a team of people passionate about healthy lifestyle, nutrition and natural food supplements. We cooperate with experts - people educated in areas related to nutrition and application of natural pro-health prophylaxis methods.

Please, Share this article on:

The original text taken from a:

Cameroonian medicinal plants: a bioactivity versus ethnobotanical survey and chemotaxonomic classification, Fidele Ntie-Kang, Lydia Likowo Lifongo, Luc Meva’a Mbaze, Nnange Ekwelle, Luc C Owono Owono, Eugene Megnassan, Philip N Judson, Wolfgang Sippl, Simon M N Efange, BMC Complementary and Alternative Medicine. The official journal of the International Society for Complementary Medicine Research (ISCMR)201313:147, DOI: 10.1186/1472-6882-13-147